Journal Article

Whole Genome Analysis of the <i>OsGRF</i> Gene Family Encoding Plant-Specific Putative Transcription Activators in Rice (<i>Oryza sativa</i> L.)

Dongsu Choi, Jeong Hoe Kim and Hans Kende

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 7, pages 897-904
Published in print July 2004 | ISSN: 0032-0781
Published online July 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch098
Whole Genome Analysis of the OsGRF Gene Family Encoding Plant-Specific Putative Transcription Activators in Rice (Oryza sativa L.)

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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OsGRF1 (Oryza sativa GROWTH-REGULATING FACTOR1) is a rice gene encoding a putative novel transcriptional regulator. We identified and characterized eleven homologs of OsGRF1 in the rice genome. All twelve OsGRF proteins have two highly conserved regions, the QLQ (Gln, Leu, Gln) and WRC (Trp, Arg, Cys) domains, and sequences reminiscent of transcription factors. OsGRF genes were preferentially expressed in young and growing tissues, and applied gibberellic acid (GA3) enhanced the expression of seven OsGRF genes. In situ hybridization showed high levels of OsGRF1 transcripts in the shoot apical meristem and in cells surrounding the vasculature of the intercalary meristem. In a GAL4-based yeast assay, the C-terminal region of OsGRF1 was found to have transactivation activity. These results indicate that OsGRF1 acts as a transcriptional activator. Based on the in situ expression pattern of OsGRF1, we postulate that it may be involved in regulating vegetative growth in rice.

Keywords: Keywords: Gibberellin — In situ hybridization — Oryza sativa — Transcription activation.; Abbreviations: GA, gibberellin; GA3, gibberellic acid.

Journal Article.  4435 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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