Journal Article

The Accumulation of α-Zein in Transgenic Tobacco Endosperm is Stabilized by Co-expression of β-Zein

Craig E. Coleman, Paula Randall Yoho, Sonja Escobar and Masahiro Ogawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 7, pages 864-871
Published in print July 2004 | ISSN: 0032-0781
Published online July 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch104
The Accumulation of α-Zein in Transgenic Tobacco Endosperm is Stabilized by Co-expression of β-Zein

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  • Biochemistry
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The cysteine-poor α-zein is the major prolamin storage protein fraction in maize endosperm and is localized in the interior of protein bodies with δ-zein, whereas the hydrophobic cysteine-rich β- and γ-zein are found on the exterior of the PB. In transgenic tobacco endosperm expressing zein genes, α-zein was unstable unless co-expressed with γ-zein. Here we showed that α-zein was also stabilized by β-zein. Small accretions of α- and β-zeins, similar in appearance to maize protein bodies, were localized to the endoplasmic reticulum within tobacco endosperm cells. The zein proteins were also localized to protein storage vacuoles in a more dispersed pattern, suggesting that they were transported there after they were post-translationally sequestered into the ER.

Keywords: Keywords: Endosperm — Maize — Protein-body — Transgenic — Tobacco — Zein.; Abbreviations: dap, days after pollination; ER, endoplasmic reticulum; PSV, protein storage vacuole; rER, rough endoplasmic reticulum.

Journal Article.  5093 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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