Journal Article

Contribution of the Plasma Membrane and Central Vacuole in the Formation of Autolysosomes in Cultured Tobacco Cells

Kanako Yano, Sumiko Matsui, Tomohiro Tsuchiya, Masayoshi Maeshima, Natsumaro Kutsuna, Seiichiro Hasezawa and Yuji Moriyasu

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 7, pages 951-957
Published in print July 2004 | ISSN: 0032-0781
Published online July 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch105
Contribution of the Plasma Membrane and Central Vacuole in the Formation of Autolysosomes in Cultured Tobacco Cells

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  • Biochemistry
  • Molecular and Cell Biology
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Autolysosomes accumulate in tobacco cells cultured under sucrose starvation conditions in the presence of a cysteine protease inhibitor. We characterized these plant autolysosomes using fluorescent dyes and green fluorescent protein (GFP). Observation using the endocytosis markers, FM4-64 and Lucifer Yellow CH, suggested that there is a membrane flow from the plasma membrane to autolysosomes. Using these dyes as well as GFP-AtVam3p, sporamin-GFP and gamma-VM23-GFP fusion proteins as markers of the central vacuole, we found transport of components of the central vacuole to autolysosomes. Thus endocytosis and the supply from the central vacuole may contribute to the formation of autolysosomes.

Keywords: Keywords: Autolysosome — Autophagy — Central vacuole — Endocytosis — FM4-64 — Green fluorescent protein.; Abbreviation: GFP, green fluorescent protein.

Journal Article.  4163 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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