Journal Article

The <i>ABNORMAL GAMETOPHYTES</i> (<i>AGM</i>) Gene Product of <i>Arabidopsis</i> Demonstrates a Role in Mitosis During Gamete Development

Anna-Marie Sorensen, Sandra Kroeber and Heinz Saedler

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 7, pages 905-913
Published in print July 2004 | ISSN: 0032-0781
Published online July 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch106
The ABNORMAL GAMETOPHYTES (AGM) Gene Product of Arabidopsis Demonstrates a Role in Mitosis During Gamete Development

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Screening a T-DNA mutagenized population of Arabidopsis thaliana for reduced seed set and segregation distortion led to the isolation of the abnormal gametophytes (agm) mutant. Homozygous plants were never recovered, but heterozygous plants showed mitotic defects during gametogenesis resulting in approximately 50% abortion of both the male and female gametes. Isolation of the genomic sequence flanking the co-segregating T-DNA element led to the identification of a gene located on chromosome 5, predicted to encode a transmembrane protein. BLAST homology searches identified two homologous proteins that are not redundant, as is clear from the existence of the agm mutant. Unexpectedly, expression studies using the β-glucuronidase reporter gene suggest that AGM and its closest Arabidopsis homolog are mostly expressed in cells undergoing mitosis. Thus, AGM is not a gametophytic gene as originally speculated on the basis of segregation distortion, but rather classified as an essential gene crucial to the process of mitosis in plants.

Keywords: Keywords: Arabidopsis — Gametogenesis — Semi-sterility — Mitosis.

Journal Article.  7256 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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