Journal Article

The <i>virescent-2</i> Mutation Inhibits Translation of Plastid Transcripts for the Plastid Genetic System at an Early Stage of Chloroplast Differentiation

Hiroki Sugimoto, Kensuke Kusumi, Yuzuru Tozawa, Junshi Yazaki, Naoki Kishimoto, Shoshi Kikuchi and Koh Iba

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 8, pages 985-996
Published in print August 2004 | ISSN: 0032-0781
Published online August 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch111
The virescent-2 Mutation Inhibits Translation of Plastid Transcripts for the Plastid Genetic System at an Early Stage of Chloroplast Differentiation

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The rice virescent-2 mutant (v2) is temperature conditional and develops chlorotic, chloroplast-deficient leaves at the restrictive temperature. In the v2 mutant, plastid-encoded proteins involved in photosynthesis and plastid transcriptional regulation were not detectable at any time during chloroplast differentiation. However, the plastid transcripts for these two classes of proteins behaved differently in the mutant, with those for the plastid transcription/translation apparatus accumulating to wild-type levels and those for photosynthetic apparatus being suppressed. Polysome analysis showed that translation of the plastid transcripts encoding the plastid transcription/translation apparatus was blocked at an early stage of chloroplast differentiation. Accumulation of transcripts of nuclear-encoded photosynthetic genes, such as cab and rbcS, was strongly suppressed in the mutant at later stages of chloroplast differentiation, whereas transcripts of genes for the plastid transcription apparatus, such as OsRpoTp and OsSIG2A, accumulated to abnormally high levels at these stages. These results suggest that activation of the plastid translation machinery at an early stage of chloroplast differentiation is important for triggering the transmission of information about plastid developmental state to the nucleus, which in turn is required for the induction of nuclear-encoded chloroplast proteins at later stages of chloroplast differentiation.

Keywords: Keywords: Chloroplast development — Nuclear-encoded plastid RNA polymerase (NEP) — Plastid-encoded plastid RNA polymerase (PEP) — Rice — Translational control — viresent-2.; Abbreviations: CAB, chlorophyll a/b-binding protein; DDT, dithiothreitol; EGTA, ethylene glycol bis(β-aminoethylether)-N,N,N′,N′-tetraacetic acid; EST, expressed sequence tags; LHCPII, chlorophyll a/b binding protein of light harvesting complex II; v2, virescent-2; NEP, nuclear-encoded plastid RNA polymerase; PEP, plastid-encoded plastid RNA polymerase; SB, shoot base; L4, unemerged fourth leaf; L3LS, leaf sheath of the third leaf; L3L, basal half of blade of the third leaf; L3U, upper half of blade of the third leaf.

Journal Article.  8089 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.