Journal Article

The Acceptor Availability at Photosystem I and ABA Control Nuclear Expression of 2-Cys Peroxiredoxin-A in <i>Arabidopsis thaliana</i>

Margarete Baier, Elke Ströher and Karl-Josef Dietz

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 8, pages 997-1006
Published in print August 2004 | ISSN: 0032-0781
Published online August 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch114
The Acceptor Availability at Photosystem I and ABA Control Nuclear Expression of 2-Cys Peroxiredoxin-A in Arabidopsis thaliana

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The redox-regulated 2-Cys peroxiredoxin-A (2CPA) promoter, which drives expression of a dominant chloroplast antioxidant enzyme, responds to signals originating from the photosynthetic electron transport downstream of PSI. Modulation of CO2- and NO3 -reduction rates in reporter gene plants expressing glucuronidase under control of the Arabidopsis thaliana 2CPA promoter revealed that promoter activity correlates with the availability of electron acceptors at PSI. The photosynthetic redox-regulation can be simulated by oxidant and antioxidant treatments. Inhibitor studies with PD98059 and staurosporine showed that a mitogen-activated protein kinase kinase transmits the oxidative response, while the antioxidant signal is transmitted by a serine/threonine kinase. Analysis of 2CPA promoter regulation in the abscisic acid (ABA)-biosynthetic mutants aba2 and aba3 and the ABA-insensitive mutants abi1 and abi2 support a regulatory circuitry in which the redox signal cross-talks with the ABA-signaling cascade downstream of ABI1 and ABI2.

Keywords: Keywords: ABA — 2-Cys peroxiredoxin — Photosynthesis — Redox — Signaling.; Abbreviations: ABRE, ABA-response element; ACC, 1-aminocyclopropane-1-carboxylic acid; DBMIB, 3-methyl-6-isopropyl-p-benzoquinone; DCMU, 3-(3′,4′-dichlorophenyl)-1,1-dimethyl urea; Fd, ferredoxin; GlcA, glucuronic acid; GUS, β-glucuronidase; JA, jasmonic acid; LUC, luciferase; MAPK(K), mitogen-activated protein kinase (kinase); P2CPA, 2CPA promoter; PS, photosystem; PQ, plastoquinone; ROS, reactive oxygen species; SA, salicylic acid; Trx, thioredoxin.

Journal Article.  6970 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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