Journal Article

Stable Isotope Labeling of <i>Arabidopsis thaliana</i> for an NMR-Based Metabolomics Approach

Jun Kikuchi, Kazuo Shinozaki and Takashi Hirayama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 8, pages 1099-1104
Published in print August 2004 | ISSN: 0032-0781
Published online August 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch117
Stable Isotope Labeling of Arabidopsis thaliana for an NMR-Based Metabolomics Approach

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  • Biochemistry
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Nuclear magnetic resonance (NMR) will become a key technology in plant metabolomics with the use of stable isotope labeling and advanced hetero-nuclear NMR methodologies. To demonstrate the power of this approach, we performed multi-dimensional hetero-nuclear NMR analysis of metabolic movement of carbon and nitrogen nuclei in Arabidopsis thaliana. First, distinct ethanol-stress response was investigated using 13C-labeled wild type and an ethanol-hypersensitive mutant plants. Furthermore, we followed nitrogen fluxes in 15N-labeled seeds during the initiation of germination in vivo. The future role of stable isotope-labeling combined with advanced hetero-nuclear NMR in plant metabolomics is discussed.

Keywords: Keywords: Arabidopsis thaliana — Nuclear magnetic resonance — Plant metabolomics — Stable isotope labeling.; Abbreviations: FT, Fourier transform; GABA, γ-aminobutyric acid; HSQC, hetero-nuclear single quantum coherence spectroscopy; MS, mass spectroscopy; NMR, nuclear magnetic resonance.

Journal Article.  3736 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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