Journal Article

Expression Analysis of the NgORF13 Promoter during the Development of Tobacco Genetic Tumors

Makiko Udagawa, Seishiro Aoki and Kunihiko Syono

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 8, pages 1023-1031
Published in print August 2004 | ISSN: 0032-0781
Published online August 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch123
Expression Analysis of the NgORF13 Promoter during the Development of Tobacco Genetic Tumors

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We investigated the expression pattern of the promoter of Nicotiana glauca (Ng) ORF13 in the hybrids between N. glauca and N. langsdorffii harboring the NgORF13-β-glucuronidase (GUS) chimeric gene. The promoter of NgORF13 of N. glauca had lower activities than the promoter of RiORF13 of Agrobacterium rhizogenes agropine-type root-inducing (Ri) plasmid. However, the localization of GUS activity in the NgORF13 transgenic plants was similar to that in the RiORF13 transgenic plants. The GUS activity of NgORF13-GUS was high in genetic tumors cultured in vitro or developed spontaneously on F1 plants with aging or by wounding. The GUS activity in tumors was observed in bud primordia, vascular bundles and leaves in the buds. While the activity was lower than in tumors, NgORF13-GUS was also expressed in vascular bundles and the parenchymatous tissues in plants regenerated from tumors. Furthermore, the promoter activity of NgORF13 was induced by wounding and activated by exogenous application of methyl jasmonate. During tumorization, NgORF13 was induced at an early stage and showed expression patterns similar to both NgrolB and NgrolC whose expression were investigated by Nagata et al. (1996) Plant Cell Physiol. 37: 489–498. It is thought that Ngrol genes might be involved in the formation of genetic tumors, and, moreover, NgORF13 might work in cooperation with NgrolB and NgrolC.

Keywords: Keywords: Agrobacterium rhizogenes — Genetic tumor — β-glucuronidase — Nicotiana — ORF13 — Transgenic plant.; Abbreviations: ORF, open reading frame; GUS, β-glucuronidase; 4MU, 4-methylumbelliferone; X-Glc, 5-bromo-4-chloro-3-indolyl-β-d-glucuronide; NAA, α-naphthaleneacetic acid; MeJA, methyljasmonate.

Journal Article.  5507 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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