Journal Article

Ole e 1, the Major Allergen from Olive (<i>Olea europaea</i> L.) Pollen, Increases its Expression and is Released to the Culture Medium during in vitro Germination

Juan de Dios Alché, Mohamed M’rani-Alaoui, Antonio Jesús Castro and María Isabel Rodríguez-García

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 9, pages 1149-1157
Published in print September 2004 | ISSN: 0032-0781
Published online September 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch127
Ole e 1, the Major Allergen from Olive (Olea europaea L.) Pollen, Increases its Expression and is Released to the Culture Medium during in vitro Germination

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  • Biochemistry
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Ole e 1 is a well-characterized allergenic protein from olive pollen. This paper examines its presence and that of its transcripts during in vitro pollen germination and pollen tube growth. A significant increase of the protein was detected after the emergence of the pollen tube, whereas part of the protein was released into the culture medium throughout pollen germination. A slight increase in the number of Ole e 1 transcripts was also detected prior to the described rise in the protein level. Within the pollen tube, the allergen was localized in the subapical region, mainly in the lumen of endoplasmic reticulum cisternae. Ole e 1 was also localized extracellularly in the vicinity of the pollen tube cell wall. These findings are discussed regarding the biological role attributed to the protein during pollen hydration and pollen tube growth and in terms of their importance for the understanding of the allergenic response in humans. On the basis of recent findings for the LAT52 protein in tomato, we propose that the homologous Ole e 1 protein might participate in a similar signal transduction pathway in olive, to control pregermination and pollen tube emergence and guidance.

Keywords: Keywords: Allergen — In vitro germination — Ole e 1 — Olea europaea — Olive tree — Pollen.

Journal Article.  6578 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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