Journal Article

Characterization of a Rice Nuclear-Encoded Plastid RNA Polymerase Gene <i>OsRpoTp</i>

Kensuke Kusumi, Asanori Yara, Naoko Mitsui, Yuzuru Tozawa and Koh Iba

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 9, pages 1194-1201
Published in print September 2004 | ISSN: 0032-0781
Published online September 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch133
Characterization of a Rice Nuclear-Encoded Plastid RNA Polymerase Gene OsRpoTp

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We isolated and characterized two rice genes, OsRpoTp and OsRpoTm, that encode putative phage-type RNA polymerases. Predicted amino acid sequences showed high homology of these genes to known RpoT genes. A transient expression assay using green fluorescent protein indicated that the encoded proteins were localized to plastids and mitochondria, respectively. We demonstrated by reverse transcription-PCR experiments and immunoblot analysis that OsRpoTp expression occurred at an early stage of leaf development, prior to the transcript accumulation of the genes that were transcribed by the nuclear-encoded plastid RNA polymerase (NEP). Expression analyses of the chloroplast-deficient rice mutant, virescent-1, showed a discrepancy between OsRpoTp protein accumulation and the level of transcripts of NEP-transcribed genes. Our results suggest that NEP activation is regulated by a process after transcription, and is affected by the developmental state of chloroplast biogenesis.

Keywords: Keywords: Bacteriophage-type RNA polymerase — Chloroplast — Mitochondrion — NEP — Oryza sativa — virescent mutant.; Abbreviations: NEP, nuclear-encoded plastid RNA polymerase; PEP, plastid-encoded plastid RNA polymerase.

Journal Article.  5259 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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