Journal Article

Ectopic Expression of a UDP-Glucose:phenylpropanoid Glucosyltransferase Leads to Increased Resistance of Transgenic Tobacco Plants Against Infection with <i>Potato Virus Y</i>

Andrea Matros and Hans-Peter Mock

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 9, pages 1185-1193
Published in print September 2004 | ISSN: 0032-0781
Published online September 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch140
Ectopic Expression of a UDP-Glucose:phenylpropanoid Glucosyltransferase Leads to Increased Resistance of Transgenic Tobacco Plants Against Infection with Potato Virus Y

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Transgenic tobacco plants over-expressing a salicylate- and pathogen-inducible glucosyltransferase (TOGT) acting on various phenylpropanoids show enhanced resistance against infection with potato virus Y (PVY). The transgenic plants are characterized by a several-fold increased glucosyltransferase activity in leaves as well as in roots. Under non-infectious conditions profiles of phenylpropanoids in leaves of transgenic lines were similar to that of controls. Feeding experiments with leaf-discs demonstrated a higher capacity for glucosylation of the coumarin scopoletin. After inoculation with PVY the transgenic lines showed similar formation of necrotic leaf lesions but significantly decreased levels of virus coat-protein when compared with control plants. Thus, our results imply that the activity of TOGT and the subsequent accumulation of glucosylated coumarins represent an important step in the cascade of events resulting in confinement of viral pathogens.

Keywords: Keywords: Defence reaction — Glucosyltransferase — Phenylpropanoid — Potato virus Y — Scopolin — Tobacco.; Abbreviations: HR, hypersensitive response; PVY, potato virus Y; SA, salicylic acid; TMV, tobacco mosaic virus; TOGT, UDP-glucose:phenylpropanoid glucosyltransferase.

Journal Article.  6413 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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