Journal Article

The <i>Arabidopsis</i> Rab5 Homologs Rha1 and Ara7 Localize to the Prevacuolar Compartment

Gil-Je Lee, Eun Ju Sohn, Myong Hui Lee and Inhwan Hwang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 9, pages 1211-1220
Published in print September 2004 | ISSN: 0032-0781
Published online September 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch142
The Arabidopsis Rab5 Homologs Rha1 and Ara7 Localize to the Prevacuolar Compartment

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Rha1, an Arabidopsis Rab5 homolog, plays a critical role in vacuolar trafficking in plant cells. In this study, we investigated the localization of Rha1 and Ara7, two Arabidopsis proteins that have highly similar amino acid sequence homology to Rab5 in animal cells. Both Ara7 and Rha1 gave a punctate staining pattern and colocalized when transiently expressed as GFP- (green fluorescent protein) or small epitope-tagged forms in Arabidopsis protoplasts. In protoplasts, transiently expressed Rha1 and Ara7 colocalized with AtPEP12p and VSRAt-1, two proteins that are known to be present at the prevacuolar compartment (PVC). Furthermore, endogenous Rha1 also gave a punctate staining pattern and colocalized with AtPEP12p to the PVC. Mutations in the first and second GTP-binding motifs alter the localizations of GFP: Rha1[S24N] in the cytosol and Rha1[Q69L] in the tonoplast of the central vacuole. Also, mutations in the effector domain and the prenylation site inhibit membrane association of Rha1. Based on these results, we propose that Rha1 and Ara7 localize to the PVC and that GTP-binding motifs as well as the effector domain are important for localization of Rha1 to the PVC.

Keywords: Keywords: Arabidopsis — Localization of GFP fusion proteins — Prevacuolar compartment — Rab proteins — Small GTP-binding proteins.

Journal Article.  7981 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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