Journal Article

Inactivation of <i>ycf33</i> Results in an Altered Cyclic Electron Transport Pathway Around Photosystem I in <i>Synechocystis</i> sp. PCC6803

Masako Ohtsuka, Junko Oyabu, Yasuhiro Kashino, Kazuhiko Satoh and Hiroyuki Koike

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 9, pages 1243-1251
Published in print September 2004 | ISSN: 0032-0781
Published online September 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch147
Inactivation of ycf33 Results in an Altered Cyclic Electron Transport Pathway Around Photosystem I in Synechocystis sp. PCC6803

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ycf33 encodes a small protein with a molecular mass of 7.5 kDa and is found from cyanobacteria to higher plants. A ycf33 deletion mutant was constructed in Synechocystis sp. PCC6803 and characterized. The mutant showed a higher phycobilisome/chlorophyll ratio than the wild type and a higher photosystem II/photosystem I fluorescence ratio measured at 77 K. Under photoautotrophic conditions, the growth rates were not much different from those of the wild type. Cyclic electron transport activities around photosystem I were not much different between the wild type and the mutant. However, the effects of diphenyleneiodonium, an inhibitor of flavoprotein, on cyclic electron transport in the mutant were different from those in the wild type; it was severely inhibited in the wild type but not much in the mutant. Together with the effects of nitrite, which accepts electrons from ferredoxin via nitrite reductase and those of HgCl2, it was suggested that the pathway of cyclic electron transport is altered in the mutant.

Keywords: Keywords: Cyclic electron transport — Diphenyleneiodonium — Ferredoxin-plastoquinone reductase — Photosystem I — Synechocystis sp. PCC6803 — ycf33.; Abbreviations: DPI, diphenyleneiodonium; Fd, ferredoxin; FNR, ferredoxin-NADP+ oxidoreductase; FQR, ferredoxin-plastoquinone reductase; NDH, NAD(P)H dehydrogenase; PQ, plastoquinone.

Journal Article.  5859 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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