Journal Article

The <i>GURKE</i> Gene Encoding an Acetyl-CoA Carboxylase is Required for Partitioning the Embryo Apex into Three Subregions in <i>Arabidopsis</i>

Takahito Kajiwara, Masahiko Furutani, Ken-ichiro Hibara and Masao Tasaka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 9, pages 1122-1128
Published in print September 2004 | ISSN: 0032-0781
Published online September 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch148
The GURKE Gene Encoding an Acetyl-CoA Carboxylase is Required for Partitioning the Embryo Apex into Three Subregions in Arabidopsis

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In Arabidopsis, three major regions, which ultimately develop into the two cotyledons, the cotyledon boundaries and the shoot apical meristem (SAM), are formed at the apex of the globular stage embryo. To reveal the molecular mechanism underlying this pattern formation, we isolated a cotyledon-defective mutant from EMS mutagenized lines. This mutant completely lacks cotyledons in the most severe cases, and is allelic to gurke (gk), which was previously reported as a mutant defective in apical patterning of the embryo. To evaluate the morphological effects of the mutation in the GK gene, we investigated the expression patterns in gk embryos of SHOOT MERISTEMLESS (STM), AINTEGUMENTA (ANT) and CUP-SHAPED COTYLEDON1 (CUC1), which are markers of the SAM, cotyledons and cotyledon boundaries, respectively. Expression of all these genes largely overlapped in gk, suggesting a failure to partition the apex of the embryo into the three subregions. Enlargement of the CUC1 expression domain was also observed and may explain the inhibition of cotyledon development in gk. Moreover, we cloned the GK gene, and confirmed that it encodes ACC1, an acetyl-CoA carboxylase which catalyzes malonyl-CoA synthesis. Our results suggest that metabolites derived from malonyl-CoA are required for partitioning of the apical part of the embryo.

Keywords: Keywords: Acetyl-CoA carboxylase — Apical pattern formation — Arabidopsis thaliana — Cotyledon — Embryogenesis — gurke.; Abbreviations: EMS, ethylmethane sulfonate; RAM, root apical meristem; SAM, shoot apical meristem.

Journal Article.  3882 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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