Journal Article

Activation of Phospholipase D Induced by Hydrogen Peroxide in Suspension-cultured Rice Cells

Takeshi Yamaguchi, Shigeru Tanabe, Eiichi Minami and Naoto Shibuya

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 9, pages 1261-1270
Published in print September 2004 | ISSN: 0032-0781
Published online September 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch150
Activation of Phospholipase D Induced by Hydrogen Peroxide in Suspension-cultured Rice Cells

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Hydrogen peroxide (H2O2) (10–100 µM) induced rapid and transient accumulation of phosphatidic acid (PA) in suspension-cultured rice cells. When phospholipase activity in the cellular extract fraction prepared from rice cells treated with H2O2 was assayed in the presence of 1-butanol (0.1%), rapid and transient phosphatidylbutanol (PtdBut) formation was observed. Thus, the H2O2-activated phospholipase was concluded to be phospholipase D (PLD). Furthermore, H2O2 directly induced in vitro PLD activation in the cytosolic fraction without H2O2 treatment. In vitro and in vivo activation of PLD were completely suppressed in the presence of lavendustin A (0.05 mM), a potent inhibitor of protein tyrosine kinase. Phytoalexin biosynthesis induced by N-acetylchitooligosaccharide elicitor was enhanced in the presence of H2O2 (10–100 µM), whereas it was suppressed in the presence of tiron, a potent scavenger of O2, 1-butanol (0.1%) and lavendustin A (0.05 mM). These results indicate that H2O2-inducible PLD activation enhances signal transduction leading to phytoalexin biosynthesis in rice cells.

Keywords: Keywords: Hydrogen peroxide — Phospholipase D — Phytoalexin — Protein tyrosine kinase.; Abbreviations: DG, diacylglycerol; DGK, diacylglycerol kinase; EGTA, ethylene glycol bis-tetraacetic acid; MAP, mitogen activated protein; NBD, (7-nitro-2–1,3-benzoxadiazol-4-yl) amino; PLC, phospholipase C; PLD, phospholipase D; PA, phosphatidic acid; PAPH, phosphatidic acid phosphohydrolase; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PIP2, Phosphatidylinositol,4,5-bisphosphate; PtdBut, phosphatidylbutanol; PTK, protein tyrosine kinase; PTP, protein tyrosine phosphatase; ROS, reactive oxygen species.

Journal Article.  7371 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.