Journal Article

The Motility of <i>Chara corallina</i> Myosin was Inhibited Reversibly by 2,3-Butanedione Monoxime (BDM)

Keisuke Funaki, Ayumi Nagata, Youka Akimoto, Kiyo Shimada, Kohji Ito and Keiichi Yamamoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 9, pages 1342-1345
Published in print September 2004 | ISSN: 0032-0781
Published online September 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch154
The Motility of Chara corallina Myosin was Inhibited Reversibly by 2,3-Butanedione Monoxime (BDM)

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We studied the effects of 2,3-butanedione monoxime (BDM) on the cytoplasmic streaming of Chara corallina and on the motility of myosin prepared from the same plant to examine whether this reagent really affects the plant class XI myosin. It was found that BDM inhibited both cytoplasmic streaming and the motility of myosin at a very similar concentration range (10–100 mM). BDM introduced directly into tonoplast-free cells also inhibited cytoplasmic streaming. These results suggested that effect of BDM on cytoplasmic streaming was exerted through myosin and not through ion channels at least in Chara corallina, though a very high concentration of BDM was required.

Keywords: Keywords: 2,3-Butanedione monoxime (BDM) — Chara corallina — Cytoplasmic streaming — In vitro motility — Plant myosin.

Journal Article.  3245 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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