Journal Article

A Comprehensive Gene Expression Analysis Toward the Understanding of Growth and Differentiation of Tobacco BY-2 Cells

Ken Matsuoka, Taku Demura, Ivan Galis, Tatsuya Horiguchi, Mami Sasaki, Gen Tashiro and Hiroo Fukuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 9, pages 1280-1289
Published in print September 2004 | ISSN: 0032-0781
Published online September 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch155
A Comprehensive Gene Expression Analysis Toward the Understanding of Growth and Differentiation of Tobacco BY-2 Cells

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  • Biochemistry
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To understand how plant cell changes gene expression during cell division and after termination of cell division, we analyzed the change of gene expression during the growth of tobacco BY-2 cell lines using a cDNA microarray, which contained about 9,200 expression sequence tag fragments and corresponded to about 7,000 genes. We found that log phase cells predominantly expressed DNA/chromosome duplication gene homologs. In addition, many genes for basic transcription and translation machineries, as well as proteasomal genes, were up-regulated at the log phase. About half of the kinesin homolog genes, but not myosin homolog genes, were predominantly expressed at the dividing phase as well. In contrast, stationary phase cells expressed genes for many receptor kinases, signal transduction machineries and transcription factors. Several hundreds of genes showed differential expression after incubation of stationary phase cells with medium containing either salicylic acid or abscisic acid. These findings suggested that BY-2 cells at the stationary phase express genes for perceiving extracellular signals.

Keywords: Keywords: BY-2 — Expression sequence tag — Gene expression — Microarray — Nicotiana tabacum — Tobacco.; Abbreviations: EST, expression sequence tag; AFLP, amplified fragment length polymorphism; LED, localized expression domain; SNARE, N-ethylmaleimide-sensitive factor adaptor protein receptor.

Journal Article.  6302 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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