Journal Article

Ten Rice Peroxidases Redundantly Respond to Multiple Stresses Including Infection with Rice Blast Fungus

Katsutomo Sasaki, Takayoshi Iwai, Susumu Hiraga, Katsushi Kuroda, Shigemi Seo, Ichiro Mitsuhara, Atsushi Miyasaka, Masataka Iwano, Hiroyuki Ito, Hirokazu Matsui and Yuko Ohashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 10, pages 1442-1452
Published in print October 2004 | ISSN: 0032-0781
Published online October 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch165
Ten Rice Peroxidases Redundantly Respond to Multiple Stresses Including Infection with Rice Blast Fungus

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  • Biochemistry
  • Molecular and Cell Biology
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Class III plant peroxidases are believed to function in diverse physiological processes including disease resistance and wound response, but predicted low substrate specificities and the presence of 70 or more isoforms have made it difficult to define a specific physiological function(s) for each gene. To select pathogen-responsive POX genes, we analyzed the expression profiles of 22 rice POX genes after infection with rice blast fungus. The expression of 10 POX genes among the 22 genes was induced after fungal inoculation in both compatible and incompatible hosts. Seven of the 10 POX genes were expressed at higher levels in the incompatible host than in the compatible host 6–24 h after inoculation by which time no fungus-induced lesions have appeared. Organ-specific expression and stress-induced expression by wounding and treatment with probenazole, an agrichemical against blast fungus, jasmonic acid, salicylic acid and 1-aminocyclopropane-1-carboxylate, a precursor of ethylene, indicated that rice POXs have individual characteristics and can be classified into several types. A comparison of the amino acid sequences of POXs showed that multiple isoforms with a high sequence similarity respond to stress in different or similar ways. Such redundant responses of POX genes may guarantee POX activities that are necessary for self-defense in plant tissues against environmental stresses including pathogen infection.

Keywords: Keywords: Classification — Peroxidase — Rice — Rice blast fungus — Stress.; Abbreviations: ACC, 1-aminocyclopropane-1-carboxylate; EST, expressed sequence tag; HR; hypersensitive reaction or response, JA, jasmonic acid; PBZ, probenazole; POX, class III plant peroxidase; SA, salicylic acid; UTR, untranslated region.

Journal Article.  7407 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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