Journal Article

Ethylene Synthesis and Auxin Augmentation in Pistil Tissues are Important for Egg Cell Differentiation after Pollination in Maize

Rafal Mól, Maria Filek, Ivana Machackova and Elisabeth Matthys-Rochon

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 10, pages 1396-1405
Published in print October 2004 | ISSN: 0032-0781
Published online October 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch167
Ethylene Synthesis and Auxin Augmentation in Pistil Tissues are Important for Egg Cell Differentiation after Pollination in Maize

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The role of ethylene and auxin in stigma-to-ovule signalling was investigated in maize (Zea mays L.). Maturation of the egg cells in an ear was stimulated before actual fertilization by the application of fresh pollen grains or quartz sand to fully receptive stigmas. Ethylene emission by maize ears increased in response to those treatments. Silks and ovaries were involved in ethylene synthesis after pollen or sand was shed over the silks. The content of ethylene precursor [1-aminocyclopropane-1-carboxylic acid (ACC)] increased in both pistil parts soon after pollination. ACC rise was delayed by 4 h in the ovaries, and by 8 h in the silks after mock-pollination with sand. The auxin level increased rapidly in the silks and ovaries after pollination, and it was very high in the pollinated silks due to the high indole-3-acetic acid (IAA) content of pollen grains. IAA rise also appeared in the silks and ovaries after treatment with sand but it was delayed by 8 h. Application of ACC (10 µM) or IAA (6 µM) solutions to non-pollinated silks stimulated maturation of the egg cells. Moreover, the response of the egg cells to pollination was cancelled by l-α-(2-aminoethoxyvinyl)-glycine, α-aminoisobutyric acid or 2,3,5-triiodobenzoic acid applied to the silks before pollination. Thus ethylene synthesis and polar auxin transport in the silks pollinated with fresh pollen were necessary to evoke accelerated differentiation of the egg cells in maize ovules. Differences in pistil responses found between true- and mock-pollination suggest that signalling pathways are at least partially different for the reception of pollen grains and sand crystals on maize stigma.

Keywords: Keywords: Egg cell maturation — Growth regulators — Maize — Pollination — Stigma-to-ovary signalling — Zea mays L.; Abbreviations: ACC, 1-aminocyclopropane-1-carboxylic acid; AIB, α-aminoisobutyric acid; AVG, l-α-(2-aminoethoxyvinyl)-glycine; IAA, indole-3-acetic acid; STS, silver thiosulfate; TIBA, 2,3,5-triiodobenzoic acid.

Journal Article.  7102 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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