Journal Article

Isolation and Characterization of Novel Mutants Affecting the Abscisic Acid Sensitivity of <i>Arabidopsis</i> Germination and Seedling Growth

Noriyuki Nishimura, Tomo Yoshida, Maki Murayama, Tadao Asami, Kazuo Shinozaki and Takashi Hirayama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 10, pages 1485-1499
Published in print October 2004 | ISSN: 0032-0781
Published online October 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch171
Isolation and Characterization of Novel Mutants Affecting the Abscisic Acid Sensitivity of Arabidopsis Germination and Seedling Growth

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To gain more insight into ABA signaling mechanisms, we conducted genetic screens searching for mutants with altered ABA response in germination and post-germination growth. We isolated seven putative ABA-hypersensitive Arabidopsis mutants and named them ABA-hypersensitive germination (ahg). These mutants exhibited diminished germination or growth ability on medium supplemented with ABA. We further studied four of them: ahg1, ahg2, ahg3 and ahg4. Mapping suggested that they were new ABA-hypersensitive loci. Characterization showed that all of them had enhanced sensitivity to salinity and high osmotic stress in germinating seeds, whereas they each had distinct sugar responses. RT–PCR experiments showed that the expression patterns of the ABA-inducible genes RAB18, AtEm1, AtEm6 and ABI5 in germinating seeds were affected by these four ahg mutations, whereas those of ABI3 and ABI4 were not. ahg4 displayed slightly increased mRNA levels of several ABA-inducible genes upon ABA treatment. By contrast, ahg1 had no clear ABA-hypersensitive phenotypes in adult plants despite its strong phenotype in germination. These results suggest that ahg1, ahg2, ahg3 and ahg4 are novel ABA-hypersensitive mutants representing distinct components in the ABA response.

Keywords: Keywords: ABA analog — ABA-hypersensitive mutant — Germination — Post-germination growth — Stress response — Sugar response.; Abbreviations: ACC, 1-aminocyclopropane-1-carboxylate; BA, 6-benzylaminopurine; BL, epibrassinolide; EMS, ethyl methanesulfonate; GA, gibberellin; NAA, α-naphthalene acetic acid; RT–PCR, reverse transcription–polymerase chain reaction; SSLP, simple-sequence-length polymorphism.

Journal Article.  9538 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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