Journal Article

Spatial and Temporal Tracing of Vessel Differentiation in Young <i>Arabidopsis</i> Seedlings by the Expression of an Immature Tracheary Element-specific Promoter

Hyunjin Pyo, Taku Demura and Hiroo Fukuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 10, pages 1529-1536
Published in print October 2004 | ISSN: 0032-0781
Published online October 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch175
Spatial and Temporal Tracing of Vessel Differentiation in Young Arabidopsis Seedlings by the Expression of an Immature Tracheary Element-specific Promoter

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The vascular system is a complex tissue composed of several vascular cell types. However, little is known about the differentiation process of each vascular cell in situ. In this study, we found that the expression of the Zinnia cysteine protease 4 (ZCP4) promoter is restricted to only immature tracheary elements (TEs) in situ. Therefore, we monitored the early TE differentiation process in young Arabidopsis seedlings using a fusion gene of the ZCP4 promoter and the β-glucuronidase gene as a molecular marker. This approach revealed unique processes of vessel differentiation during early seedling development, in which discontinuous initiation of vessel element differentiation occurs at distinct regions, followed by the simultaneous differentiation of protoxylem vessels and bidirectional differentiation of metaxylem vessels to form a vessel in the plant body.

Keywords: Keywords: Arabidopsis — Cysteine protease — Metaxylem vessel — Programmed cell death — Protoxylem vessel — Tracheary element differentiation.; Abbreviations: GUS, β-glucuronidase; HAI, hour after imbibition; PCD, programmed cell death; TE, tracheary element.

Journal Article.  5667 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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