Journal Article

Radial Expansion of Root Cells and Elongation of Root Hairs of <i>Arabidopsis thaliana</i> Induced by Massive Doses of Gamma Irradiation

Toshifumi Nagata, Setsuko Todoriki and Shoshi Kikuchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 11, pages 1557-1565
Published in print November 2004 | ISSN: 0032-0781
Published online November 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch178
Radial Expansion of Root Cells and Elongation of Root Hairs of Arabidopsis thaliana Induced by Massive Doses of Gamma Irradiation

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Radial expansion of root cells and elongation of root hairs were induced within 3 d of a massive dose (3 kGy) of gamma irradiation to Arabidopsis thaliana. Because treatment with the antioxidant n-propyl gallate before irradiation suppressed these changes, gamma irradiation partially rescued the rhd2 mutant (defective in NADPH oxidase); the superoxide-generating reagent paraquat induced similar root morphogenesis. These responses appeared to be induced by the active oxygen species (AOS) generated by water radiolysis. Ethylene production was induced immediately after gamma irradiation and reached a steady level after about 2 h. Addition of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid partly induced a similar expansion of root cells and elongation of root hairs. Addition of an inhibitor of ethylene biosynthesis, aminoethoxyvinylglycine, before gamma irradiation completely suppressed the formation of abnormal structures. These results suggest that the AOS is involved in the root morphological changes through the ethylene biosynthesis induced by gamma irradiation in Arabidopsis.

Keywords: Keywords: Active oxygen species — Ethylene — Gamma irradiation — Root hair.; Abbreviations: ACC, 1-aminocyclopropane-1-carboxylic acid; AOS, active oxygen species; AVG, aminoethoxyvinylglycine; MAPKKK, mitogen-activated protein kinase kinase kinase; n-PG, n-propyl gallate.

Journal Article.  4630 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.