Journal Article

Induction of PR-1 Accumulation Accompanied by Runaway Cell Death in the <i>lsd1</i> Mutant of <i>Arabidopsis</i> is Dependent on Glutathione Levels but Independent of the Redox State of Glutathione

Kaori Senda and Ken’ichi Ogawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 11, pages 1578-1585
Published in print November 2004 | ISSN: 0032-0781
Published online November 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch179
Induction of PR-1 Accumulation Accompanied by Runaway Cell Death in the lsd1 Mutant of Arabidopsis is Dependent on Glutathione Levels but Independent of the Redox State of Glutathione

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The lesions simulating disease (lsd) mutants of Arabidopsis spontaneously develop hypersensitive-response-like lesions in the absence of pathogens. To address the function of the redox regulator glutathione in disease resistance, we examined the relationship between endogenous glutathione and PR-1 accumulation using one of these mutants, lsd1, as a disease resistance model. Lesion formation on lsd1 was suppressed by weak light and initiated by the subsequent transition to normal light. The application of buthionine sulfoximine, a specific inhibitor of glutathione biosynthesis, suppressed conditionally induced runaway cell death and expression of the PR-1 gene, suggesting that glutathione regulates the conditional cell death and PR-1 gene expression. The application of reduced (GSH) or oxidized (GSSG) glutathione to lsd1 upregulated the level of total glutathione ([GSH]+[GSSG]) accompanied by hastened accumulation of PR-1, and the basal level of total glutathione in lsd1 was higher than that in wild-type plants. The glutathione redox state defined as [GSH]/([GSH]+[GSSG]) decreased following the conditional transition, but the suppression of this decrease by the application of GSH did not inhibit the accumulation of PR-1. Taken together, conditional PR-1 accumulation in lsd1 is regulated not by the redox state but by the endogenous level of glutathione.

Keywords: Keywords: Cell death — Disease resistance — Glutathione — lsd1 — PR-1 — Redox.; Abbreviations: BSO, l-Buthionine sulfoximine; γ-ECS, γ-glutamylcysteine synthetase; GSH, reduced glutathione; GSSG, oxidized glutathione; ROS, reactive oxygen species; RT-PCR, reverse transcription-polymerase chain reaction; SA, salicylic acid.

Journal Article.  5194 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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