Journal Article

Ectopic Expression of the NtSET1 Histone Methyltransferase Inhibits Cell Expansion, and Affects Cell Division and Differentiation in Tobacco Plants

Wen-Hui Shen and Denise Meyer

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 11, pages 1715-1719
Published in print November 2004 | ISSN: 0032-0781
Published online November 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch184
Ectopic Expression of the NtSET1 Histone Methyltransferase Inhibits Cell Expansion, and Affects Cell Division and Differentiation in Tobacco Plants

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The tobacco NtSET1 gene encodes a member of the SUV39H family of histone methyltransferases. Ectopic expression of NtSET1 causes an increase in methylated histone H3 lysine 9 and abnormal chromosome segregation in tobacco suspension cells, and inhibits tobacco plant growth. Here we show that the inhibition of plant growth was caused by reduced cell expansion as well as by abnormal cell division and differentiation. We found that deletion of the C-terminally located catalytic domain of the protein abolished the ectopic effects of NtSET1 on plant growth. Our results indicate that histone H3 lysine 9 methylation is a critical mark of epigenetic control for plant development.

Keywords: Keywords: Chromatin — Epigenetics — Histone methyltransferase — Plant development — SET domain.; Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; DEX, dexamethasone; HMTase, histone methyltransferase; GFP, green fluorescent protein; H3K9, histone 3 lysine 9; OE, overexpression; SET, Su(var)-E(z)-Trithorax associated domain.

Journal Article.  2289 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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