Journal Article

Azetidine-induced Accumulation of Class I Small Heat Shock Proteins in the Soluble Fraction Provides Thermotolerance in Soybean Seedlings

Tsung-Luo Jinn, Chi-Chou Chiu, Wei-Wen Song, Yih-Ming Chen and Chu-Yung Lin

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 12, pages 1759-1767
Published in print December 2004 | ISSN: 0032-0781
Published online December 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch193
Azetidine-induced Accumulation of Class I Small Heat Shock Proteins in the Soluble Fraction Provides Thermotolerance in Soybean Seedlings

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  • Biochemistry
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Accumulation of class I small heat shock proteins (sHSPs) is induced by the proline analog, azetidine-2-carboxylic acid (Aze) in soybean seedlings to a level similar to that induced by exposure to 40°C. However, only the treatment with 10 mM Aze for 6 h and subsequently with 10 mM proline for 24 h protected the seedlings from damage during subsequent exposure to 45°C as assessed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. A chaperone activity assay showed that the purified class I sHSPs induced by Aze were functional in vitro and protected proteins from thermal denaturation. Amino acid composition analysis indicated that Aze was not incorporated into de novo synthesized class I sHSPs. Accumulation of class I sHSPs in the soluble post-ribosomal supernatant fraction was found to be important for acquisition of thermotolerance. We suggest that both the accumulation of class I sHSPs and their presence in the soluble fraction are important for establishment of thermotolerance.

Keywords: Keywords: Amino acid analog — Azetidine — Glycine max — Heat shock — Plant class I sHSPs — Thermotolerance.; Abbreviations: Aze, l-azetidine-2-carboxylic acid; HS, heat shock; sHSPs, small heat shock proteins; PRS, post-ribosomal supernatant; TTC, 2,3,5-triphenyltetrazolium chloride.

Journal Article.  6555 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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