Journal Article

A Mechanosensitive Anion Channel in <i>Arabidopsis thaliana</i> Mesophyll Cells

Zhi Qi, Akio Kishigami, Yuko Nakagawa, Hidetoshi Iida and Masahiro Sokabe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 11, pages 1704-1708
Published in print November 2004 | ISSN: 0032-0781
Published online November 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch194
A Mechanosensitive Anion Channel in Arabidopsis thaliana Mesophyll Cells

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  • Biochemistry
  • Molecular and Cell Biology
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Mechanosensitive ion channels are expected to play important roles in transducing mechanical stimuli into intracellular signals during the development and morphogenesis of higher plants. We have identified a novel mechanosensitive anion channel in the protoplast of Arabidopsis thaliana mesophyll cells by using the patch-clamp technique. The channel in the outside-out patches could be activated by positive pressure in the pipette while negative pressure had no effect. The amphipathic membrane crenator trinitrophenol, which is supposed to preferentially insert in the outer leaflet of the lipid bilayer of the plasma membrane, synergized with mechanical membrane stretch to activate the channel. These results suggest that the channel activation is mediated by a convex curvature of the plasma membrane. Therefore, activation of this channel may play an important role when cell volume is increasing during cell growth or hypo-osmotic challenge, which is accompanied by membrane stretch with increasingly convex curvature.

Keywords: Keywords: Anion channel — Arabidopsis thaliana — Cell expansion — Mechanosensitive channel — Mesophyll cell.; Abbreviations: RVD, regulatory volume decrease; TEA+, tetraethylammonium ion; TNP, trinitrophenol.

Journal Article.  3149 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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