Journal Article

Effects of Chloramphenicol on Photosynthesis, Protein Profiles and Transketolase Activity under Extremely High CO<sub>2</sub> Concentration in an Extremely-high-CO<sub>2</sub>-tolerant Green Microalga, <i>Chlorococcum littorale</i>

Akira Satoh, Norihide Kurano, Shigeaki Harayama and Shigetoh Miyachi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 12, pages 1857-1862
Published in print December 2004 | ISSN: 0032-0781
Published online December 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch196
Effects of Chloramphenicol on Photosynthesis, Protein Profiles and Transketolase Activity under Extremely High CO2 Concentration in an Extremely-high-CO2-tolerant Green Microalga, Chlorococcum littorale

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An extremely-high-CO2-tolerant alga, Chlorococcum littorale, showed high quantum efficiency of PSII (ΦII) in the light at 40% CO2, as well as at 5% CO2. However, ΦII decreased greatly when chloramphenicol (CAP) was added at 40% CO2, while no such decrease was observed at 5% CO2. Cycloheximide showed no effect on ΦII at either 5% or 40% CO2. The amount of a 76 kDa polypeptide (p76) on SDS–PAGE decreased markedly in the presence of CAP at 40% CO2 but not at 5% CO2. A partial amino acid sequence of p76 was 71–100% identical (10–14 identical residues out of 14 amino acids determined) to those of transketolases (TKLs) reported in higher plants and a cyanobacterium. In agreement with these observations, the TKL activity in C. littorale was decreased by CAP at 40% CO2, but not at 5% CO2. The transient decrease in TKL activity caused by CAP under 40% CO2 was well correlated with that in ΦII. These results indicate that the addition of CAP directly or indirectly influences the stability of TKL in C. littorale at 40% CO2, but not at 5% CO2, and that photosynthetic activity was reduced by a decrease in TKL activity.

Keywords: Keywords: Chlorococcum littorale — High-CO2 acclimation — Photosynthesis — Protein synthesis — Transketolase.; Abbreviations: CAP, chloramphenicol; CBB, Coomassie Brilliant blue; CHI, cycloheximide; ΦII, quantum efficiency of PSII; PCV, packed cell volume; TKL, transketolase.

Journal Article.  4153 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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