Journal Article

Development of Q-chromosome-specific DNA Markers in Tobacco and Their Use for Identification of a Tobacco Monosomic Line

Takahiro Tezuka, Katsura Onosato, Saori Hijishita and Wataru Marubashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 12, pages 1863-1869
Published in print December 2004 | ISSN: 0032-0781
Published online December 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch204
Development of Q-chromosome-specific DNA Markers in Tobacco and Their Use for Identification of a Tobacco Monosomic Line

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  • Biochemistry
  • Molecular and Cell Biology
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We developed seven Q-chromosome-specific DNA markers in Nicotiana tabacum by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis using two hybrid lines, and we were able to identify tobacco monosomic plants among F1 progeny derived from the cross N. tabacum Haplo-Q × N. tabacum cv. Samsun NN using Q-chromosome-specific DNA markers. Based on the results, we discuss the roles of the Q chromosome in embryo sac development and embryogenesis. Here, we propose a new method for identifying DNA markers for a particular chromosome in the genus Nicotiana.

Keywords: Keywords: Chromosome-specific DNA marker — Embryogenesis — Embryo sac development —Monosomic — PCR — Tobacco.; Abbreviations: CTAB, cetyltrimethylammonium bromide; DAPI, 4′,6-diamino-2-phenylindole dihydrochloride; FISH, fluorescence in situ hybridization; GISH, genomic in situ hybridization; ISSR, inter-simple sequence repeat; PCD, programmed cell death; RAPD, random amplified polymorphic DNA; RFLP, restriction fragment length polymorphism; SCAR, sequence characterized amplified region; STS, sequence tagged site.

Journal Article.  3821 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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