Journal Article

<i>Arabidopsis</i> Plasma Membrane Proteomics Identifies Components of Transport, Signal Transduction and Membrane Trafficking

Erik Alexandersson, Gerhard Saalbach, Christer Larsson and Per Kjellbom

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 11, pages 1543-1556
Published in print November 2004 | ISSN: 0032-0781
Published online November 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch209
Arabidopsis Plasma Membrane Proteomics Identifies Components of Transport, Signal Transduction and Membrane Trafficking

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In order to identify integral proteins and peripheral proteins associated with the plasma membrane, highly purified Arabidopsis plasma membranes from green tissue (leaves and petioles) were analyzed by mass spectrometry. Plasma membranes were isolated by aqueous two-phase partitioning, which yields plasma membrane vesicles with a cytoplasmic-side-in orientation and with a purity of 95%. These vesicles were turned inside-out by treatment with Brij 58 to remove soluble contaminating proteins enclosed in the vesicles and to remove loosely bound contaminating proteins. In total, 238 putative plasma membrane proteins were identified, of which 114 are predicted to have transmembrane domains or to be glycosyl phosphatidylinositol anchored. About two-thirds of the identified integral proteins have not previously been shown to be plasma membrane proteins. Of the 238 identified proteins, 76% could be classified according to function. Major classes are proteins involved in transport (17%), signal transduction (16%), membrane trafficking (9%) and stress responses (9%). Almost a quarter of the proteins identified in the present study are functionally unclassified and more than half of these are predicted to be integral.

Keywords: Keywords: Arabidopsis — Integral membrane proteins — Peripheral membrane proteins — Plasma membrane proteins — Proteome.; Abbreviations: AHA, plasma membrane H+-ATPase; H+-PPiase, H+-inorganic pyrophosphatase; PIP, plasma membrane intrinsic protein; TIP, tonoplast intrinsic protein; SYP, syntaxin.

Journal Article.  9533 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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