Journal Article

Identification and Subcellular Localization of Two Solanesyl Diphosphate Synthases from <i>Arabidopsis thaliana</i>

Luo Jun, Ryoichi Saiki, Kei Tatsumi, Tsuyoshi Nakagawa and Makoto Kawamukai

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 12, pages 1882-1888
Published in print December 2004 | ISSN: 0032-0781
Published online December 2004 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pch211
Identification and Subcellular Localization of Two Solanesyl Diphosphate Synthases from Arabidopsis thaliana

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Two solanesyl diphosphate synthases, designated SPS1 and SPS2, which are responsible for the synthesis of the isoprenoid side chain of either plastoquinone or ubiquinone in Arabidopsis thaliana, were identified. Heterologous expression of either SPS1 or SPS2 allowed the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coli. SPS1–GFP was found to localize in the ER while SPS2–GFP localized in the plastid of tobacco BY-2 cells. These two different subcellular localizations are thought to be the reflection of their roles in solanesyl diphosphate synthesis in two different parts: presumably SPS1 and SPS2 for the side chains of ubiquinone and plastoquinone, respectively.

Keywords: Keywords: CoenzymeQ — Solanesyl diphosphate synthase — Ubiquinone.

Journal Article.  3726 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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