Journal Article

Peroxisomal Localization of Sulfite Oxidase Separates it from Chloroplast-based Sulfur Assimilation

Katharina Nowak, Nora Luniak, Christina Witt, Yvonne Wüstefeld, Andreas Wachter, Ralf R. Mendel and Robert Hänsch

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 12, pages 1889-1894
Published in print December 2004 | ISSN: 0032-0781
Published online December 2004 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pch212
Peroxisomal Localization of Sulfite Oxidase Separates it from Chloroplast-based Sulfur Assimilation

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Recently, we isolated the sulfite oxidase (SO) gene from Arabidopsis thaliana and characterized the purified SO protein. The purpose of the present study was to determine the subcellular localization of this novel plant enzyme. Immunogold electron-microscopic analysis showed the gold labels nearly exclusively in the peroxisomes. To verify this finding, green fluorescent protein was fused to full-length plant SO including the putative peroxisomal targeting signal 1 (PTS1) ‘SNL’ and expressed in tobacco leaves. Our results showed a punctate fluorescence pattern resembling that of peroxisomes. Co-labelling with MitoTracker-Red excluded that the observed fluorescence was due to mitochondrial sorting. By investigation of deleted or mutated PTS1, no functional peroxisomal targeting signal 2 (PTS2) could be detected in plant SO. This conclusion is supported by expression studies in Pichia pastoris mutants with defined defects either in PTS1- or PTS2-mediated peroxisomal import.

Keywords: Keywords: CLSM — GFP — Peroxisomal targeting signal — Reporter gene expression — Transmission electron microscopy; Abbreviations: CaMV, cauliflower mosaic virus; CLSM, confocal laser scanning microscopy; GFP, green fluorescent protein, Moco, molybdenum cofactor; PTS, peroxisomal targeting signal; SO, sulfite oxidase; YFP, yellow fluorescent protein.

Journal Article.  4036 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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