Journal Article

Ultraviolet-B Sensitivities in Japanese Lowland Rice Cultivars: Cyclobutane Pyrimidine Dimer Photolyase Activity and Gene Mutation

Mika Teranishi, Yutaka Iwamatsu, Jun Hidema and Tadashi Kumagai

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 12, pages 1848-1856
Published in print December 2004 | ISSN: 0032-0781
Published online December 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch215
Ultraviolet-B Sensitivities in Japanese Lowland Rice Cultivars: Cyclobutane Pyrimidine Dimer Photolyase Activity and Gene Mutation

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

There is a cultivar difference in the response to ultraviolet-B (UVB: 280–320 nm) in rice (Oryza sativa L.). Among Japanese lowland rice cultivars, Sasanishiki, a leading Japanese rice cultivar, is resistant to the damaging effects of UVB while Norin 1, a close relative, is less resistant. We found previously that Norin 1 was deficient in cyclobutane pyrimidine dimer (CPD) photorepair ability and suggested that the UVB sensitivity in rice depends largely on CPD photorepair ability. In order to verify that suggestion, we examined the correlation between UVB sensitivity and CPD photolyase activity in 17 rice cultivars of progenitors and relatives in breeding of UV-resistant Sasanishiki and UV-sensitive Norin 1. The amino acid at position 126 of the deduced amino acid sequence of CPD photolyase in cultivars including such as Norin 1 was found to be arginine, the CPD photolyase activities of which were lower. The amino acid at that position in cultivars including such as Sasanishiki was glutamine. Furthermore, cultivars more resistant to UVB were found to exhibit higher photolyase activities than less resistant cultivars. These results emphasize that single amino acid alteration from glutamine to arginine leads to a deficit of CPD photolyase activity and that CPD photolyase activity is one of the main factors determining UVB sensitivity in rice.

Keywords: Keywords: Cyclobutane pyrimidine dimer (CPD) — CPD photolyase — Gene mutation — Rice (Oryza sative L.) — UVB radiation — UVB sensitivity.; Abbreviations: Arg, arginine; BSA, bovine serum albumin; CPD, cyclobutane pyrimidine dimer; CTAB, cetyl trimethyl ammonium bromide; DTT, dithiothreitol; Gln, glutamine, NIL, near-isogenic line; PAR, photosynthetic active radiation; (6–4) pp, pyrimidine (6–4) pyrimidone photoproducts; QTL, quantitative trait loci; UVB, UV light with wavelengths from 280 to 320 nm.

Journal Article.  7021 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.