Journal Article

Low Concentrations of Propyzamide and Oryzalin Alter Microtubule Dynamics in <i>Arabidopsis</i> Epidermal Cells

Masayoshi Nakamura, Kuniko Naoi, Tsubasa Shoji and Takashi Hashimoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 9, pages 1330-1334
Published in print September 2004 | ISSN: 0032-0781
Published online September 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pch300
Low Concentrations of Propyzamide and Oryzalin Alter Microtubule Dynamics in Arabidopsis Epidermal Cells

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Previous studies showed that sub-micromolar concentrations of the microtubule-targeting herbicide propyzamide cause a right-handed helical arrangement of cortical microtubule arrays and left-handed twisting in elongating Arabidopsis epidermal cells. When seedlings were grown in the presence of 1–2 µM propyzamide or 50–100 nM oryzalin, we show that microtubules spent more time in a paused state in which they exhibited little net change in length. The drug treatment also resulted in slower growth and shortening velocities, increased catastrophe, and an overall decrease in microtubule turnover. A reduction in microtubule dynamic turnover may underlie the drug-induced rearrangement of cortical arrays.

Keywords: Keywords: Arabidopsis — Dynamic instability — Microtubules — Oryzalin — Propyzamide.; Abbreviations: GFP, green fluorescent protein; GFP-TUB, a fusion protein comprising GFP and Arabidopsis β-tubulin 6; MT, microtubule.

Journal Article.  2941 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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