Journal Article

Cell Cycle Dependence of Elicitor-induced Signal Transduction in Tobacco BY-2 Cells

Yasuhiro Kadota, Takashi Watanabe, Shinsuke Fujii, Yutaka Maeda, Ryoko Ohno, Katsumi Higashi, Toshio Sano, Shoshi Muto, Seiichiro Hasezawa and Kazuyuki Kuchitsu

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 1, pages 156-165
Published in print January 2005 | ISSN: 0032-0781
Published online January 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci008
Cell Cycle Dependence of Elicitor-induced Signal Transduction in Tobacco BY-2 Cells

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The molecular links between the cell cycle and defense responses in plants are largely unknown. Using synchronized tobacco BY-2 cells, we analyzed the cell cycle dependence of elicitor-induced defense responses. In synchronized cultured apoaequorin-expressing cells, the increase in cytosolic free Ca2+ induced by a proteinaceous elicitor, cryptogein, was greatly suppressed during the G2 and M phases in comparison with G1 or S phases. Treatment with cryptogein during the G1 or S phases also induced biphasic (rapid/transient and slow/prolonged) responses in activation of mitogen-activated protein kinases (MAPKs) and production of reactive oxygen species (ROS). In contrast, elicitor treatment during the G2 or M phases induced only a rapid and transient phase of MAPK activation and ROS production. Their slow and prolonged phases as well as expression of defense-related genes, cell cycle arrest and cell death were induced only after the cell cycle progressed to the G1 phase; removal of the elicitor before the start of the G1 phase inhibited these responses. These results suggest that although cryptogein recognition occurred at all phases of the cell cycle, the recognition during the S or G1 phases, but not at the G2 or M phases, induces the prolonged activation of MAPKs and the prolonged production of ROS, followed by cell cycle arrest, accumulation of defense-related gene transcripts and cell death. Elicitor signal transduction depends on the cell cycle and is regulated differently at each phase.

Keywords: Keywords: Cell cycle arrest — Cell death — Elicitor — Synchronized culture — Tobacco BY-2 cells; Abbreviations: [Ca2+]cyt, cytosolic free Ca2+ concentration; MAPK, mitogen-activated protein kinase; MCLA, 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo[1,2-α]pyrazin-3-one; •O2–, superoxide anion radical; ROS, reactive oxygen species; SIPK, salicylic acid-induced protein kinase; WIPK, wounding-induced protein kinase.

Journal Article.  6762 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.