Journal Article

Characterization of Full-length Enriched Expressed Sequence Tags of Stress-treated Poplar Leaves

Tokihiko Nanjo, Norihiro Futamura, Mitsuru Nishiguchi, Tomohiro Igasaki, Kazuo Shinozaki and Kenji Shinohara

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 45, issue 12, pages 1738-1748
Published in print December 2004 | ISSN: 0032-0781
Published online December 2004 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci009
Characterization of Full-length Enriched Expressed Sequence Tags of Stress-treated Poplar Leaves

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  • Biochemistry
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  • Plant Sciences and Forestry

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Poplar, whose genome is the first to be sequenced among woody plants, is a favorable model for plant biologists to enable them to understand molecular processes of growth, development and responses to environmental stimuli in trees. The sequence will allow the development of a strategy for improving environmental stress tolerance in forest trees. In this study, we have generated a full-length enriched cDNA library from leaves of axenically grown poplar (Populus nigra var. italica) subjected to environmental stress treatments by dehydration, high salinity, chilling, heat, abscisic acid (ABA) and H2O2. We sequenced >30,000 expressed sequence tags (ESTs) from the cDNA library and consequently collected approximately 4,500 non-redundant clones. We further analyzed cDNAs encoding an ERF/AP2-domain transcription factor which is specific in plants and plays an important role under stress. Thirteen candidates containing the ERF/AP2 domain were found within our EST resource. Some of them showed stress-responsive gene expression. We report here the first collection of full-length enriched stress-related ESTs of poplar and discuss environmental stress responses of forest trees in the light of comparative genomics.

Keywords: Keywords: Environmental stress — ERF/AP2-domain transcription factor — Expressed sequence tags — Full-length cDNA — Populus nigra.; Abbreviations: CBF, C-repeat-binding factor; DIG, digoxigenin; DRE, dehydration-responsive element; DREB, DRE-binding transcription factor; ERF, ethylene-responsive factor; EST, expressed sequence tag; KOGs, eukaryotic clusters of orthologous groups of proteins; PTA, Paracel Transcript Assembler; RAFL, RIKEN Arabidopsis Full-Length; UTR, untranslated region.

Journal Article.  6895 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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