Journal Article

Involvement of the Chloroplast Signal Recognition Particle cpSRP43 in Acclimation to Conditions Promoting Photooxidative Stress in <i>Arabidopsis</i>

Markus Klenell, Shigeto Morita, Mercedes Tiemblo-Olmo, Per Mühlenbock, Stanislaw Karpinski and Barbara Karpinska

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 1, pages 118-129
Published in print January 2005 | ISSN: 0032-0781
Published online January 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci010
Involvement of the Chloroplast Signal Recognition Particle cpSRP43 in Acclimation to Conditions Promoting Photooxidative Stress in Arabidopsis

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  • Molecular and Cell Biology
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In this study, we have investigated the role of the CAO gene (coding for the chloroplast recognition particle cpSRP43) in the protection against and acclimation to environmental conditions that promote photooxidative stress. Deficiency of cpSRP43 in the Arabidopsis mutant chaos has been shown previously to lead to partial loss of a number of proteins of the photosystem II (PSII) antennae. In addition, as reported here, mutant plants have lower growth rates and reduced lignin contents under laboratory conditions. However, chaos seedlings showed significantly higher tolerance to photooxidative stress under both tightly controlled laboratory conditions and highly variable conditions in the field. This greater tolerance of chaos plants was manifested in less photooxidative damage together with faster growth recovery in young seedlings. It was also associated with a lower production of H2O2, lower ascorbate levels and less induction of ascorbate peroxidases. Under field conditions, chaos exhibited better overall photosynthetic performance and had higher survival rates. Expression of the CAO gene may be regulated by a light-dependent chloroplastic redox signalling pathway, and was inhibited during acclimation to high light and chilling temperatures, simultaneously with induction of ascorbate peroxidases. It is concluded that the presence/absence of the CAO gene has an impact on photo-produced H2O2, lignification in the hypocotyls and on the plant’s susceptibility to photooxidative stress. Therefore, regulation of the CAO gene may be part of the plant’s system for acclimation to high light and chilling temperatures.

Keywords: Keywords: Abiotic stress tolerance — Ascorbate peroxidase — Hydrogen peroxide — Photooxidative stress — Signal recognition particle.; Abbreviations: APX, ascorbate peroxidase; cpSRP, chloroplast signal recognition particle; DAB, 3,3′-diaminobenzidine; DBMIB, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone; DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea; DW, dry weight; EEE, excess excitation energy; ELIP, early light-inducible protein; Fv/Fm, the quantum efficiency of open photosystem II centres; FW, fresh weight; ΦPSII, proxy of quantum yield of electron transport from water through PSII; LHC, light-harvesting complex; NPQ, non-photochemical quenching; qp, estimation of PSII centres in the open state; ROS, reactive oxygen species; VAZ, violaxanthin–antheraxantin–zeaxanthin.

Journal Article.  8711 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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