Journal Article

Light-induced Wilting and its Molecular Mechanism in Epicotyls of Dark-germinated Pea (<i>Pisum sativum</i> L.) Seedlings

Noémi Erdei, Csengele Barta, Éva Hideg and Béla Böddi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 1, pages 185-191
Published in print January 2005 | ISSN: 0032-0781
Published online January 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci012
Light-induced Wilting and its Molecular Mechanism in Epicotyls of Dark-germinated Pea (Pisum sativum L.) Seedlings

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Possible mechanisms behind the light-induced wilting of dark-germinated pea (Pisum sativum L.) epicotyls were studied. Illumination with photosynthetically active radiation caused a fast turgor loss and wilting in the middle segments of the epicotyls accompanied by accumulation of water in the intercellular cavities. During this process, room temperature fluorescence emission spectra showed gradual bleaching of porphyrin-type pigments, which was lessened by incubating the epicotyls with excess ascorbate before illumination. Detection of singlet oxygen and lipid peroxidation products in the illuminated epicotyls suggested the occurrence of porphyrin-photosenzitized membrane damage as a cause of disordered water status and sequential wilting.

Keywords: Keywords: Chlorophyllous pigments — Lipid peroxidation — Pea epicotyl — Photooxidative — Protochlorophyllide — Singlet oxygen.; Abbreviations: Chlide, chlorophyllide; DanePy, 3-[N-(β-diethylaminoethyl)-N-dansyl]aminomethyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrole; MDA, malondialdehyde; PAR, photosynthetically active radiation; Pchl, protochlorophyllide ester; Pchlide, protochlorophyllide; PLB, prolamellar body; ROS, reactive oxygen species; TBA, thiobarbituric acid; TBARS, thiobarbituric acid-reactive substances; TEP, 1,1,3,3-tetraethoxypropane.

Journal Article.  4540 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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