Journal Article

Involvement of Extracellular Dilignols in Lignification During Tracheary Element Differentiation of Isolated <i>Zinnia</i> Mesophyll Cells

Naohito Tokunaga, Norikazu Sakakibara, Toshiaki Umezawa, Yasuko Ito, Hiroo Fukuda and Yasushi Sato

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 1, pages 224-232
Published in print January 2005 | ISSN: 0032-0781
Published online January 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci017
Involvement of Extracellular Dilignols in Lignification During Tracheary Element Differentiation of Isolated Zinnia Mesophyll Cells

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

During differentiation of isolated Zinnia mesophyll cells into tracheary elements (TEs), lignification on TEs progresses by supply of monolignols not only from TEs themselves but also from surrounding xylem parenchyma-like cells through the culture medium. However, how lignin polymerizes from the secreted monolignols has not been resolved. In this study, we analyzed phenol compounds in culture medium with reversed-phase HPLC, gas chromatography–mass spectrometry and nuclear magnetic resonance spectrometry, and found 12 phenolic compounds including coniferyl alcohol and four dilignols, i.e. erythro-guaiacylglycerol-β-coniferyl ether, threo-guaiacylglycerol-β-coniferyl ether, dehydrodiconiferyl alcohol and pinoresinol, in the medium in which TEs were developing. Coniferyl alcohol applied to TE-inductive cultures during TE formation rapidly disappeared from the medium, and caused a sudden increase in dilignols. Addition of the dilignols promoted lignification of TEs in which monolignol biosynthesis was blocked by an inhibitor of phenylalanine anmmonia-lyase (PAL), l-α-aminooxy-β-phenylpropionic acid (AOPP). These results suggested that dilignols can act as intermediates of lignin polymerization.

Keywords: Keywords: Coniferyl alcohol — Dilignol — Lignification — Tracheary element — Zinnia elegans.; Abbreviations: AOPP, l-α-aminooxy-β-phenylpropionic acid; CA, coniferyl alcohol; CD, coniferaldehyde; DMSO, dimethylsulfoxide; GC-MS, gas chromatography–mass spectrometry; NMR, nuclear magnetic resonance; PAL, phenylalanine ammonia-lyase; SA, sinapyl alcohol; TE, tracheary element.

Journal Article.  6012 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.