Journal Article

A Novel Vesicle Derived Directly from Endoplasmic Reticulum is Involved in the Transport of Vacuolar Storage Proteins in Rice Endosperm

Hideyuki Takahashi, Yuhi Saito, Tomoya Kitagawa, Shigeto Morita, Takehiro Masumura and Kunisuke Tanaka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 1, pages 245-249
Published in print January 2005 | ISSN: 0032-0781
Published online January 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci019
A Novel Vesicle Derived Directly from Endoplasmic Reticulum is Involved in the Transport of Vacuolar Storage Proteins in Rice Endosperm

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We found novel vesicles derived from rough endoplasmic reticulum (ER) in rice endosperm. The novel vesicles had characteristic structures different from that of the ER-derived protein body type I and the Golgi-derived dense vesicles. Immunocytochemical analysis revealed that the novel vesicles are derived directly from the aggregates of vacuolar storage proteins in the rough ER. In addition, BiP, an ER-resident molecular chaperone, was localized in the novel vesicles, but also in protein storage vacuoles (PSVs). These results suggest that the novel vesicles mediate transport of vacuolar storage proteins directly from the ER to PSVs in rice endosperm.

Keywords: Keywords: Endoplasmic reticulum — Endosperm — Protein body — Rice — Seed storage protein — Vesicle transport; Abbreviations: C-ER, cisternal endoplasmic reticulum; DAF, days after flowering; ER, endoplasmic reticulum; PAC vesicle, precursor-accumulating vesicle; PB, protein body; PB-ER, protein body endoplasmic reticulum; PSV, protein storage vacuole.

Journal Article.  3415 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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