Journal Article

Characterization of a member of the AN subfamily, IAN, from <i>Ipomoea nil</i>

Kiu-Hyung Cho, Takayuki Shindo, Gyung-Tae Kim, Eiji Nitasaka and Hirokazu Tsukaya

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 1, pages 250-255
Published in print January 2005 | ISSN: 0032-0781
Published online January 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci020
Characterization of a member of the AN subfamily, IAN, from Ipomoea nil

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ANGUSTIFOLIA (AN) is the first C-terminal binding protein (CtBP) gene from plants and controls leaf width and pattern of trichome branching in Arabidopsis thaliana (L.) Heynh. We characterized an ortholog of AN from Ipomoea nil (L.) Roth (Japanese morning glory) and designated it Ipomoea nil’s AN (IAN). IAN is a single-copy gene in the genome and is expressed ubiquitously in various organs of I. nil. IAN contains not only a D2-HDH motif, which is highly conserved within the CtBP family, but also LXCXE, NLS and PEST motifs, which are specific to the AN subfamily. The expression of IAN cDNA driven by the cauliflower mosaic virus 35S promoter restored a defect in leaf expansion in the leaf width direction in the angustifolia-1 (an-1) mutant of Arabidopsis, suggesting that IAN retains a common function with AN. In contrast, the complementation by IAN of a defect in the trichome branching pattern on the leaf surface of the an-1 mutant was less effective than that observed for leaf shape. These results suggest that the mechanisms by which AN regulates leaf width and trichome branching are separable.

Keywords: Keywords: ANGUSTIFOLIA — Arabidopsis thaliana — IAN — Ipomoea nil — Leaf shape — Trichome; Abbreviations: CaMV, cauliflower mosaic virus; CtBP, C-terminal binding protein; D2-HDH, NAD-dependent 2-hydroxy acid dehydrogenase; EST, expressed sequence tag; NLS, nuclear localization signal; RT–PCR, reverse transcription–polymerase chain reaction.

Journal Article.  3471 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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