Journal Article

Phosphorylation of Sphingoid Long-chain Bases in <i>Arabidopsis</i>: Functional Characterization and Expression of the First Sphingoid Long-chain Base Kinase Gene in Plants

Hiroyuki Imai and Hideki Nishiura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 2, pages 375-380
Published in print February 2005 | ISSN: 0032-0781
Published online February 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci023
Phosphorylation of Sphingoid Long-chain Bases in Arabidopsis: Functional Characterization and Expression of the First Sphingoid Long-chain Base Kinase Gene in Plants

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Sphingoid long-chain base (LCB) kinase catalyzes the phosphorylation of LCBs to form LCB 1-phosphates. Based on sequence identity to murine sphingosine kinase (mSPHK1), we cloned and characterized the first plant LCB kinase gene from Arabidopsis (AtLCBK1). Using recombinant AtLCBK1 protein from Escherichia coli cells, we confirmed that the enzyme specifically phosphorylated d-erythro-dihydrosphingosine (DHS), but not N-acetyl-DHS or d-threo-DHS. AtLCBK1 also phosphorylated d-erythro-sphingosine, trans-4, trans-8-sphingadienine and phytosphingosine. We found that AtLCBK1 mRNA is highly expressed in flowers. AtLCBK1 transcripts were slightly increased by low humidity or abscisic acid treatments, suggesting that AtLCBK1 is constitutively expressed under these treatments.

Keywords: ABA; Dehydration stress; Long-chain base; Phytosphingosine; Sphingosine kinase; T87 cells; DHS, dihydrosphingosine (sphinganine); DHS-1P, dihydrosphingosine 1-phosphate; EST, expressed sequence tag; GPCR, G-protein-coupled receptor; LCB, sphingoid long-chain base; LCBK, sphingoid long-chain base kinase; ORF, open reading frame; PHS, phytosphingosine (4-hydroxysphinganine); PHS-1P, phytosphingosine 1-phosphate; RT–PCR, reverse transcription–polymerase chain reaction; SPH, sphingosine (trans-4-sphingenine); SPH-1P, sphingosine 1-phosphate; SPHK, sphingosine kinase

Journal Article.  3521 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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