Journal Article

Genome-wide Identification of the Rice Calcium-dependent Protein Kinase and its Closely Related Kinase Gene Families: Comprehensive Analysis of the <i>CDPKs</i> Gene Family in Rice

Takayuki Asano, Naoki Tanaka, Guangxiao Yang, Nagao Hayashi and Setsuko Komatsu

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 2, pages 356-366
Published in print February 2005 | ISSN: 0032-0781
Published online February 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci035
Genome-wide Identification of the Rice Calcium-dependent Protein Kinase and its Closely Related Kinase Gene Families: Comprehensive Analysis of the CDPKs Gene Family in Rice

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In plants, calcium acts as a universal second messenger in various signal transduction pathways. The plant-specific calcium-dependent protein kinases (CDPKs) play important roles regulating downstream components of calcium signaling. We conducted a genome-wide analysis of rice CDPKs and identified 29 CDPK genes and eight closely related kinase genes, including five CDPK-related kinases (CRKs), one calcium and calmodulin-dependent protein kinase (CCaMK) and two phosphoenolpyruvate (PEP) carboxylase kinase-related kinases (PEPRKs). The mRNA splicing sites of the rice CDPKs, CRKs and PEPRKs (but not OsCCaMK) are highly conserved, suggesting that these kinases are derived from a common ancestor. RNA gel blot analyses revealed that the majority of rice CDPK genes exhibited tissue-specific expression. Expression of OsCPK9 was elevated in seedlings infected by rice blast, indicating that this gene plays an important role in signaling in response to rice blast treatment. Our genomic and bioinformatic analyses will provide an important foundation for further functional dissection of the rice CDPK gene family.

Keywords: Calcium-dependent protein kinase; Gene expression; Gene structure; Rice; CCaMK, calcium and calmodulin-dependent protein kinase; CDPK, calcium-dependent protein kinase; CRK, CDPK-related kinase; EST, expressed sequence tag; PEPRK, phosphoenolpyruvate (PEP) carboxylase kinase-related kinase; RT–PCR, reverse transcription–polymerase chain reaction; SPK, seed-specific protein kinase; UTR, untranslated region

Journal Article.  6937 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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