Journal Article

Study of the Constitutively Active Form of the α Subunit of Rice Heterotrimeric G Proteins

Katsuyuki Oki, Yukiko Fujisawa, Hisaharu Kato and Yukimoto Iwasaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 2, pages 381-386
Published in print February 2005 | ISSN: 0032-0781
Published online February 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci036
Study of the Constitutively Active Form of the α Subunit of Rice Heterotrimeric G Proteins

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  • Biochemistry
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We used site-directed mutagenesis to engineer two constitutively active forms of the α subunit of a rice heterotrimeric G protein. The recombinant proteins produced from these novel cDNAs had GTP-binding activity but no GTPase activity. A chimeric gene for a constitutively active form of the α subunit was introduced into the rice mutant d1, which is defective for the α-subunit gene. All the transformants essentially showed a wild-type phenotype compared with normal cultivars, although seed sizes were substantially increased and internode lengths also showed some increase.

Keywords: Constitutively active form; Heterotrimeric G protein; Rice; Site-directed mutagenesis; Transgenic plant; GA, gibberellin; GUS, β-glucuronidase

Journal Article.  4195 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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