Journal Article

Metabolic Flux Analysis of the Phenylpropanoid Pathway in Elicitor-treated Potato Tuber Tissue

Fumio Matsuda, Keiko Morino, Rieko Ano, Masaki Kuzawa, Kyo Wakasa and Hisashi Miyagawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 3, pages 454-466
Published in print March 2005 | ISSN: 0032-0781
Published online March 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci042
Metabolic Flux Analysis of the Phenylpropanoid Pathway in Elicitor-treated Potato Tuber Tissue

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The effects of β-1,3-oligosaccharide elicitor on the metabolism of phenylpropanoids in potato tuber were analyzed quantitatively, by monitoring the time-dependent changes in the levels of seven compounds. The elicitor treatment caused an increase in the pool size of octopamine and tyramine amides (N-p-coumaroyloctopamine, N-feruloyloctopamine, N-p-coumaroyltyramine and N-feruloyltyramine), as well as a decrease in that of chlorogenic acid and putrescine amides (caffeoylputrescine and feruloylputrescine). An analysis of metabolic flux using stable isotope labeling and liquid chromatography–spectrometry (LC–MS) detection clearly demonstrated that the changes in the pool size of these compounds were correlated with the changes in their flux for biosynthesis (J in) upon elicitor treatment. The increase in J in in the cases of octopamine and tyramine amides was accompanied by an increase in flux for the transformation (J out), indicating a rapid turnover of these compounds in the elicitor-treated tuber tissue. The result of the flux analysis indicated that the actual activation of the biosynthesis of octopamine and tyramine amides after the elicitor treatment was greater than that estimated from the changes in their levels in the potato tissue. These findings suggest that these amide compounds and their metabolic derivatives play an important role in the defense-related metabolism of phenylpropanoids in potato.

Keywords: Hydroxycinnamic acid conjugates; Metabolic flux analysis; Phenylpropanoid pathway; Plant defense response; Solanum tuberosum; CafP, caffeoylputrescine; CGA, chlorogenic acid; 4CL, 4-hydroxycinnamic acid:CoA ligase; CST, hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase; CQT, hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferase; FO, N-feruloyloctopamine; FP, feruloylputrescine; FT, N-feruloyltyramine; LC–MS, liquid chromatography–mass spectrometry; PAL, phenylalanine ammonia lyase; p-CO, N-p-coumaroyloctopamine; p-CT, N-p-coumaroyltyramine; Phe*, l-phenyl-d5-alanine; TFA, trifluoroacetic acid; THT, tyramine hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase

Journal Article.  9039 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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