Journal Article

Cellular Internalization of Fluorescent Proteins via Arginine-rich Intracellular Delivery Peptide in Plant Cells

Microsugar Chang, Jyh-Ching Chou and Han-Jung Lee

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 3, pages 482-488
Published in print March 2005 | ISSN: 0032-0781
Published online March 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci046
Cellular Internalization of Fluorescent Proteins via Arginine-rich Intracellular Delivery Peptide in Plant Cells

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The protein delivery across cellular membranes or compartments is limited by low biomembrane permeability because of the hydrophobic characteristics of cell membranes. Usually the delivery processes utilize passive protein channels or active transporters to overcome the membrane impediment. In this report, we demonstrate that arginine-rich intracellular delivery (AID) peptide is capable of efficiently delivering fused fluorescent proteins unpreferentially into different plant tissues of both tomato (a dicot plant) and onion (a monocot plant) in a fully bioactive form. Thus, cellular internalization via AID peptide can be a powerful tool characterized by its simplicity, non-invasion and high efficiency to express those bioactive proteins in planta or in plant cells in vivo. This novel method may alternatively provide broader applications of AID chimera in plants without the time-consuming transgenic approaches.

Keywords: Cellular internalization; Green fluorescent protein; Protein transduction; Recombinant protein; Transgenic plant; AID, arginine-rich intracellular delivery; GFP, green fluorescent protein; HIV-1, human immunodeficiency virus type 1; IPTG, isopropyl-β-d-thiogalactopyranoside; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide; NEM, N-ethylmaleimide; PTD, protein transduction domain; R9, nona-arginine; RFP, red fluorescent protein

Journal Article.  4026 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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