Journal Article

Isolation of a Novel Carotenoid-rich Protein in <i>Cyanophora paradoxa</i> that is Immunologically Related to the Light-harvesting Complexes of Photosynthetic Eukaryotes

Heather M. Rissler and Dion G. Durnford

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 3, pages 416-424
Published in print March 2005 | ISSN: 0032-0781
Published online March 2005 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pci054
Isolation of a Novel Carotenoid-rich Protein in Cyanophora paradoxa that is Immunologically Related to the Light-harvesting Complexes of Photosynthetic Eukaryotes

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Novel eukaryotic chlorophyll–carotenoid proteins have evolved at least twice following the origin of the plastid and include the widely distributed integral membrane light-harvesting complexes (LHCs) and the dinoflagellate-specific soluble peridinin–chlorophyll proteins. In the glaucophytes, homologs of these proteins are reportedly absent. We have identified a novel carotenoid-rich protein (CRP) in the glaucophyte Cyanophora paradoxa that is 28 kDa and immunologically related to the family of LHCs. CRP is associated with the thylakoid membrane, though it can be removed by stringent washes, suggesting that there are probably significant structural differences between CRP and the LHCs. CRP co-localizes with a zeaxanthin-rich thylakoid membrane fraction that also contains β-carotene, chlorophyll and an unidentified carotenoid. Despite this, we found no evidence for carotenoid–chlorophyll energy transfer in the isolated complex, suggesting that light harvesting may not be a primary function. The presence of CRP in C. paradoxa is evidence for the evolution of a novel pigment-binding protein in the glaucophytes.

Keywords: Cyanophora paradoxa; Light-harvesting complex; Pigment-binding protein; Plastid evolution; Zeaxanthin; CBR protein, carotenoid biosynthesis-related protein; CRP, carotenoid-rich protein; ELIP, early light-inducible protein; FCP, fucoxanthin–chlorophyll protein; FP, free pigment; FV/FM, maximum yield of PSII; GF, green fraction; HL, high light; LHC, light-harvesting complex; LL, low light; MSR, membrane-spanning region; PBS, phycobilisomes; PCP, peridinin–chlorophyll protein; SEP, stress-enhanced proteins; TLC, thin-layer chromatography; WSC protein, water-soluble chlorophyll protein; YL, yellow fraction

Journal Article.  6443 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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