Journal Article

The Tomato Photomorphogenetic Mutant, <i>aurea</i>, is Deficient in Phytochromobilin Synthase for Phytochrome Chromophore Biosynthesis

Takuya Muramoto, Chitose Kami, Hideo Kataoka, Naoko Iwata, Philip J. Linley, Keiko Mukougawa, Akiho Yokota and Takayuki Kohchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 4, pages 661-665
Published in print April 2005 | ISSN: 0032-0781
Published online April 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci062
The Tomato Photomorphogenetic Mutant, aurea, is Deficient in Phytochromobilin Synthase for Phytochrome Chromophore Biosynthesis

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The aurea mutants of tomato have been widely used as phytochrome-deficient mutants for photomorphogenetic and photobiological studies. By expressed sequence tag (EST)-based screening of sequence databases, we found a tomato gene that encodes a protein homologous to Arabidopsis HY2 for phytochromobilin synthase catalyzing the last step of phytochrome chromophore biosynthesis. The tomato protein expressed in Escherichia coli showed phytochromobilin synthase activity. The corresponding loci in all aurea mutants tested have nucleotide substitutions, deletions or DNA rearrangements. These results indicate that aurea is a mutant of phytochromobilin synthase in tomato. We also discuss a phylogenetic analysis of phytochromobilin synthases in the bilin reductase family.

Keywords: Aurea; Photomorphogenesis; Phytochrome; Phytochromobilin; AU, AUREA; BV, biliverdin; Cph1, cyanobacterial phytochrome 1; EST, expressed sequence tag; GST, glutathione S-transferase; PCB, phycocyanobilin; PcyA, phycocyanobilin:ferredoxin oxidoreductase; PΦB, phytochromobilin; YG-2, YELLOW-GREEN-2

Journal Article.  3507 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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