Journal Article

Isolation and Characterization of cDNAs Encoding an Enzyme with Glucosyltransferase Activity for <i>cyclo</i>-DOPA from Four O’clocks and Feather Cockscombs

Nobuhiro Sasaki, Katsuhiro Wada, Takatoshi Koda, Kichiji Kasahara, Taiji Adachi and Yoshihiro Ozeki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 4, pages 666-670
Published in print April 2005 | ISSN: 0032-0781
Published online April 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci064
Isolation and Characterization of cDNAs Encoding an Enzyme with Glucosyltransferase Activity for cyclo-DOPA from Four O’clocks and Feather Cockscombs

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cDNAs encoding an enzyme with UDP-glucose:cyclo-DOPA 5-O-glucosyltransferase activity were isolated from four o’clocks and feather cockscombs. Phylogenetic analysis of the amino acid sequences deduced from the cDNAs show that they represent a single subclade distinct from those of other phenylpropanoid and flavonoid glucosyltransferases. Changes in the amount of transcripts of the cDNA in four o’clocks correlated with the accumulation of betanin during flower development. The cDNAs isolated here were candidates for the gene of the enzyme involved in another pathway of betacyanin biosynthesis via glucosylation at the cyclo-DOPA step rather than at the betanidin step.

Keywords: Betacyanin; Celosia cristata; cyclo-DOPA; Glucosyltransferase; Mirabilis jalapa; cyclo-DOPA, 5,6-dihydroxyindoline-2-carboxylic acid; cDOPA5GT, UDP-glucose:cyclo-DOPA 5-O-glucosyltransferase; DOPA, dihydroxyphenylalanine

Journal Article.  3037 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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