Journal Article

Analysis of the Function of the Photoreceptors Phytochrome B and Phytochrome D in <i>Nicotiana plumbaginifolia</i> and <i>Arabidopsis thaliana</i>

Aurora Piñas Fernández, Patricia Gil, Ildiko Valkai, Ferenc Nagy and Eberhard Schäfer

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 5, pages 790-796
Published in print May 2005 | ISSN: 0032-0781
Published online May 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci073
Analysis of the Function of the Photoreceptors Phytochrome B and Phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana

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  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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To investigate the mechanism of phytochrome action in vivo, NtPHYB, AtPHYB and phyD:green fluorescent protein (GFP) were overexpressed in Nicotiana plumbaginifolia and Arabidopsis thaliana. The expression of 35S:NtPHYB:GFP and 35S:AtPHYB:GFP complemented the tobacco hgl2 and Arabidopsis phyB-9 mutations, whereas the 35S:AtPHYD:GFP only rescued the hgl2 mutant. All three fusion proteins are transported into the nucleus in all genetic backgrounds. These data indicate that AtPHYD:GFP is biologically active and functions as the main red light receptor in transgenic tobacco, and establish an experimental system for the functional analysis of this elusive photoreceptor in vivo.

Keywords: Light signalling; Nicotiana plumbaginifolia; Phytochrome; AtPHY, generic or collective symbol for Arabidopsis thaliana phytochrome protein, apoprotein; GFP, green fluorescent protein; NtPHY, generic or collective symbol for Nicotiana tabacum phytochrome protein, apoprotein; phy, generic or collective symbols for fully assembled, photoreversible chromoprotein (holoprotein) with chromophore covalently attached to PHY apoprotein; phy, generic or collective symbol for mutant phytochrome genes; 35S, cauliflower mosaic virus 35S promoter; UVB, ultraviolet B wavelength

Journal Article.  4404 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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