Journal Article

Differential Expression Pattern of C<sub>4</sub> Bundle Sheath Expression Genes in Rice, a C<sub>3</sub> Plant

Mika Nomura, Tomonori Higuchi, Yuji Ishida, Shozo Ohta, Toshihiko Komari, Nobuyuki Imaizumi, Mitsue Miyao-Tokutomi, Makoto Matsuoka and Shigeyuki Tajima

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 5, pages 754-761
Published in print May 2005 | ISSN: 0032-0781
Published online May 2005 | e-ISSN: 1471-9053 | DOI:
Differential Expression Pattern of C4 Bundle Sheath Expression Genes in Rice, a C3 Plant

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PCK) are specifically expressed in bundle sheath cells (BSCs) in NADP-ME-type and PCK-type C4 plants, respectively. Unlike the high activities of these enzymes in the green leaves of C4 plants, their low activities have been detected in the leaves of C3 plants. In order to elucidate the differences in the gene expression system between C3 and C4 plants, we have produced chimeric constructs with the β-glucuronidase (GUS) reporter gene under the control of the maize NADP-Me (ZmMe) or Zoysia japonica Pck (ZjPck) promoter and introduced these constructs into rice. In leaves of transgenic rice, the ZmMe promoter directed GUS expression not only in mesophyll cells (MCs) but also in BSCs and vascular cells, whereas the ZjPck promoter directed GUS expression only in BSCs and vascular cells. Neither the ZjPck nor ZmMe promoters induced GUS expression due to light. In rice leaves, the endogenous NADP-Me (OsMe1) was expressed in MCs, BSCs and vascular cells, whereas the rice Pck (OsPck1) was expressed only in BSCs and vascular cells. Taken together, the results obtained from transgenic rice demonstrate that the expression pattern of ZmMe or ZjPck in transgenic rice was reflected by that of its counterpart gene in rice.

Keywords: C4 plant; NADP-malic enzyme; Phosphoenolpyruvate carboxykinase; Promoter analysis; Rice; BSC, bundle sheath cell; MC, mesophyll cell; ME, malic enzyme; PCK, phosphoenolpyruvate carboxykinase; RT–PCR, reverse transcription–Polymerase chain reaction

Journal Article.  5400 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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